Vibriostatic effects of probiotic Bacillus licheniformis Dahb1 and its molecular phylogeny resolved through RAPD markers |
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Authors: | Baranabas David Jayaseelan Baskaralingam Vaseeharan Athisuyambulingam Maharajan Sathappan Shanthi Gopalakrishnan Vinoj |
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Affiliation: | 1. Department of Animal Health and Management, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India 2. PG & Research Department of Zoology, Khadir Mohideen College, Adirampattinam, 614701, Thanjavur Dist, Tamil Nadu, India
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Abstract: | The in vitro vibriostatic effects of probiotic Bacillus licheniformis strains (Dahb1 to Dahb7) from both wild and commercial sources were evaluated against pathogenic Vibrios isolated from shrimp hatcheries and farms. Agar antagonism assay results showed that, out of seven B. licheniformis strains, strain Dahb1 showed the biggest inhibitory zone (6–12 mm) tested against 162 isolates of Vibrio spp. viz., V. harveyi (53 isolates), V. anguillarum (42 isolates), V. vulnificus (31 isolates) and Photobacterium damselae ssp. damselae (36 isolates) obtained from Penaeus monodon culture hatcheries and ponds. The genetic status of these seven strains were analyzed through randomly amplified polymorphic DNA analysis using 18 random primers. Of the 18 primers studied, only 6 generated repeatable polymorphic DNA bands with sizes ranging from 250 to 1,000 bp in seven isolates of B. licheniformis. The dendrogram generated from resolved gel profiles showed two major branches with three clusters. The results of the present study allow us to conclude that B. licheniformis Dahb1 can be used as an effective probiotic to control Vibrios. Field studies are needed to evaluate probiotic efficiency to control diseases in aquatic organisms. |
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