Low metabolic activity of biofilm formed by Enterococcus faecalis isolated from healthy humans and wild mallards (Anas platyrhynchos) |
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Authors: | W?odzimierz Meissner Tomasz A Jarzembowski Hanna Rzyska Claudia Botelho Anna Pa?ubicka |
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Institution: | 1. Avian Ecophysiology Unit, Department of Vertebrate Ecology and Zoology, University of Gdańsk, ul. Wita Stwosza 59, 80-308, Gdańsk, Poland 2. Department of Medical Microbiology, Medical University of Gdańsk, ul. Do Studzienki 38, 80-227, Gdańsk, Poland 3. IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal 4. Department of Laboratory and Microbiological Diagnostics, Ko?cierzyna Medical Center, ul. Piechowskiego 36, 83-400, Ko?cierzyna, Poland
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Abstract: | It is widely known that Enterococcus faecalis virulence is related to its biofilm formation. Although Enterococci are common commensal organisms of the gastrointestinal tract, the difference between commensal and pathogen strains remain unclear. In this study, we compare the biochemical profile of the biofilms formed by two groups of medical and two groups of commensal strains. The medical strains were isolated as pathogens from infections of urinary tract and other infections (wounds, pus and bedsores), and the commensal strains were taken from faeces of healthy volunteers and faeces of wild mallards (Anas platyrhynchos) living in an urban environment. The properties of biofilms formed by medical and commensal strains differed significantly. Commensal strains showed lower metabolic activity and glucose uptake and higher biofilm biomass than the medical ones. Consistent with glucose uptake experiments, we found that the glucose dehydrogenase gene was more expressed in medical strains. These results indicate that higher metabolic activity and lower protein concentration of E. faecalis cells within biofilms are formed during infections. |
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