K+-induced ion-exchanges trigger trypsin activation in pancreas acinar zymogen granules |
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Authors: | Yang Kai Ding Yong-Xue Chin Wei-Chun |
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Institution: | Department of Biological Sciences, Florida State University, Tallahassee, FL 32310, USA. |
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Abstract: | Trypsin premature activation has been thought to be a key event in the initiation phase of acute pancreatitis. Here we test a hypothesis that a sustained increase of cytosolic Ca(2+) concentration (Ca(2+)](C)) can trigger K(+) influx into pancreas acinar zymogen granules (ZGs) via a Ca(2+)-activated K(+) channel (K(Ca)), and this influx of K(+) then mobilizes bound-Ca(2+) by K(+)/Ca(2+) ion-exchange to increase free Ca(2+) concentration in the ZGs (Ca(2+)](G)) and release bound-H(+) by K(+)/H(+) ion-exchange to decrease the pH in ZGs (pH(G)). Both the increase of Ca(2+)](G) and the decrease of pH(G) will facilitate trypsinogen autoactivation and stabilize active trypsin inside ZGs that could lead to acute pancreatitis. The experimental results are consistent with our hypothesis, suggesting that K(+) induced ion-exchanges play a critical role in the initiation of trypsin premature activation in ZGs. |
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Keywords: | Initiation mechanism Acute pancreatitis Zymogen granules Ion exchange K+/Ca2+ K+/H+ |
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