The enhancing effects of anion channel blockers on sperm activation by bicarbonate |
| |
| Authors: | Yutaka Tajima Naomichi Okamura |
| |
| Affiliation: | Division of Biochemistry, Institute of Basic Medial Sciences, University of Tsukuba, Tsukuba Japan |
| |
| Abstract: | We found that anion channel blockers such as phosphotungstate and 4,4′-diisothiocyanatostilbene-2-2′-disulfonate (DIDS)_enhanced HCO3?-induced activation on porcine epididymal sperm. In the presence of these compounds, HCO3? increased the motility, respiration rate and especially the cAMP content of the sperm to a greater extent than did HCO3? alone. The enhancing effects were not observed in the absence of HCO3?, but were evident when the concentration of HCO3? was low. These compounds did not significantly alter the intracellular pH and did inhibit the adenylate cyclase activity of the sperm plasma membrane. When these compounds were added to sperm homogenate with ATP, the cAMP formed was reduced compared to the control. In addition, these compounds inhibited both the SO42? influx and efflux of the sperm. From these results, we conclude that the anion channel blockers tested principally inhibit the efflux of endogenous HCO3? derived from metabolic CO2, so that HCO3? accumulates intracellularly and stimulates the adenylate cyclase of the sperm. |
| |
| Keywords: | Anion channel blocker Anion transport Adenylate cyclase Bicarbonate Cyclic AMP Sperm DIDS 4,4′-diisothiocyanatostilbene-2,2′-disulfonate SITS 4-acetamido-4′-isothiocyanatostilbene-2,2′-disulfonate PLP pyridoxal 5-phosphate G1P glucose 1-phosphate BCECF 2′7′-bis(carboxyethyl)carboxyfluorescein BCECF-AM tetraacetoxy-methyl ester of BCECF intracellular pH PTA phosphotungstate STA silicotungstate DBP PDE phosphodiesterase |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
