Elevated oxalate oxidase activity is correlated with Al-induced plasma membrane injury and root growth inhibition in young barley roots |
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Authors: | Ladislav Tamás Marta Šimonovi ová Jana Huttová Igor Mistrík |
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Institution: | (1) Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 14, SK-84523 Bratislava, Slovakia |
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Abstract: | In order to characterise the possible mechanisms involved in Al toxicity some functional characteristics were analysed in
young barley (Hordeum vulgare L.) seedlings cultivated between moistened filter paper. Transfer of germinated barley seeds into hydroponic culture system
caused significant stress, which was manifested by root-growth inhibition and elevated Evans blue uptake of root tips. Hydroponics
caused stress unabled the analysis of Al-induced stress in the young barley roots during the first day of cultivation. Several
(3–4) days are required for adaptation of barley seedlings to hydroponics in spite of strong aeration of the medium. Using
filter paper compared to cultivation in solution application of much higher Al concentrations were required to inhibit root
growth. Al-induced root growth inhibition, Al uptake, damage of plasma-membrane (PM) permeability of root cells, as well as
elevated oxalate oxidase - OxO (EC 1.2.3.4) activity were significantly correlated. While 1 mM Al concentration had no effect
on barley roots growing on filter paper, 5 to 100 mM Al concentration inhibited root growth, enhanced cell death and induced
oxalate oxidase activity with increasing intensity. The time course analysis of OxO gene expression and OxO activity showed
that 10 mM Al increased OxO activity as soon as 3 h after exposure of roots to Al reaching its maximum at about 18 h after
Al application. These results indicate that expression of OxO is activated very early after exposure of barley to Al, suggesting
its role in oxidative stress and subsequent cell death caused by Al toxicity in plants. |
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Keywords: | Al-uptake cell death root growth inhibition |
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