| 共培养对小鼠囊胚质量及其表观遗传修饰的影响 |
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| 引用本文： | 王保垒,赵禹,刘军,权富生,华松,张涌. 共培养对小鼠囊胚质量及其表观遗传修饰的影响[J]. 生物工程学报, 2009, 25(5): 733-738 |
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| 作者姓名： | 王保垒 赵禹 刘军 权富生 华松 张涌 |
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| 作者单位： | 西北农林科技大学动物医学院生物工程研究所,杨凌,712100 |
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| 基金项目： | 国家高技术研究发展计划(863计划)(No.2004AA213072)资助~~ |
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| 摘 要： | 本研究探讨了共培养对小鼠囊胚质量及其表观遗传修饰的影响。将小鼠的受精卵体外随机分别置于含颗粒细胞(试验组I)、输卵管上皮细胞(试验组Ⅱ)、输卵管组织块(试验组Ⅲ)的KSOM培养液中作为试验组进行共培养,同时设立对照组A(体外培养,仅含KSOM)和对照组B(体内培养)。比较各组受精卵的卵裂率和囊胚发育率;并应用碘化丙啶和Hoechest333258对囊胚进行染色,利用ICM/TE值评价各组胚胎体外发育的质量;同时将囊胚进行免疫荧光染色,观察其基因组甲基化和组蛋白乙酰化的水平。结果表明,与对照组A相比,试验组的卵裂率和囊胚发育率均有显著提高(P<0.05);同时其囊胚细胞数目及内细胞团细胞数与滋养层细胞数比值(ICM/TE)值均显著高于对照组A(P<0.05);各试验组囊胚基因组甲基化水平与对照组A差异不显著(P>0.05),但各体外培养组均与对照组B差异显著(P<0.05);试验组组蛋白乙酰化水平与对照组A、B差异均不显著(P>0.05)。共培养能够有效促进小鼠胚胎的体外发育,提高囊胚的发育质量,但是仍不能克服由体外培养造成的基因组甲基化异常。
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| 关 键 词： | 小鼠 共培养 基因组甲基化 组蛋白乙酰化 表观遗传 |
| 收稿时间： | 2008-11-11 |
Co-culture of mouse blastocysts and their epigenetic modification |
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| Baolei Wang,Yu Zhao,Jun Liu,Fusheng Quan,Song Hua and Yong Zhang. Co-culture of mouse blastocysts and their epigenetic modification[J]. Chinese journal of biotechnology, 2009, 25(5): 733-738 |
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| Authors: | Baolei Wang Yu Zhao Jun Liu Fusheng Quan Song Hua Yong Zhang |
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| Affiliation: | Bioengineering Institute, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China;Bioengineering Institute, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China;Bioengineering Institute, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China;Bioengineering Institute, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China;Bioengineering Institute, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China;Bioengineering Institute, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China |
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| Abstract: | |
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| Keywords: | mouse co-culture genome methylation histone acetylation epigenetic |
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