Fractionation of RNA from tetrahymena by affinity chromatography on poly-u-sepharose

Summary Exponential growing Tetrahymena pyriformis organisms were labelled with (³H) uridine or (³H) adenosine. The labelled RNA was extracted and isolated by affinity chromatography on poly-uridylic-acid Sepharose and further analysed by means of sucrose gradient centrifugation and RNase digestion.Experimental evidence proved the existence of RNase resistant poly adenylic-acid fragments in the RNA of Tetrahymena cells. This poly adenylic-acid segment has a sedimentation rate of 4-5 S and would be localised in the 10-12S region of the RNA which is probably the m-RNA.Supported by Stiftung Volkswagenwerk Research Grant No.112273.