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Controlled release mechanisms of spontaneously forming unilamellar vesicles |
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| Authors: | Mu-Ping Nieh John Katsaras Xiaoyang Qi |
| Institution: | a Canadian Neutron Beam Centre, Steacie Institute for Molecular Sciences, National Research Council Canada, Chalk River Laboratory, Chalk River, Ontario, Canada K0J 1J0 b Guelph-Waterloo Physics Institute and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada N1G 2W1 c Department of Physics, Brock University, 500 Glenridge Avenue, St. Catharines, Ontario, Canada L2S 3A1 d Division of Human Genetics, Cincinnati Children Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA |
| Abstract: | Spontaneously forming small unilamellar vesicles (SULVs) are easy to prepare and show great promise for use in delivering therapeutic payloads. We report of SULVs made up of the ternary phospholipid mixture, dimyristoyl-phosphatidylcholine (DMPC), dihexanoyl-phosphatidylcholine (DHPC) and dimyristoyl-phosphatidylglycerol (DMPG), which have been characterized by small angle neutron scattering (SANS). These low-polydispersity (0.14-0.19) SULVs range in size (i.e., radius) from 110 to 215 Å and are capable of entrapping, and subsequently releasing, hydrophilic molecules (e.g., fluorescent dyes and quenchers) in a controlled fashion over two different temperature ranges. The low-temperature release mechanism involves the SULVs transforming into discoidal micelles, with an onset temperature (To) of ~ 32 °C, while the high-temperature release mechanism is more gradual, presumably the result of defects formed through the continuous dissolution of DHPC into solution. Both of these mechanisms differ from other, previously reported thermosensitive liposomes. |
| Keywords: | Spontaneously forming unilamellar vesicles Small angle neutron scattering Thermosensitive Controlled release Phospholipid DMPC DHPC DMPG Low-polydispersity Fluorescence |
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