Structural stability and properties of three isoforms of the major light-harvesting chlorophyll a/b complexes of photosystem II |
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Authors: | Yajie Zhang Cheng Liu Shuang Liu Tingyun Kuang |
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Institution: | a Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Beijing, 100093, China b Institut für Allgemeine Botanik, Johannes-Gutenberg-Universität Mainz, D-55099 Mainz, Germany |
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Abstract: | Three isoforms of the major light-harvesting chlorophyll (Chl) a/b complexs of photosystem II (LHCIIb) in the pea, namely, Lhcb1, Lhcb2, and Lhcb3, were obtained by overexpression of apoprotein in Escherichia coli and by successfully refolding these isoforms with thylakoid pigments in vitro. The sequences of the protein, pigment stoichiometries, spectroscopic characteristics, thermo- and photostabilities of different isoforms were analysed. Comparison of their spectroscopic properties and structural stabilities revealed that Lhcb3 differed strongly from Lhcb1 and Lhcb2 in both respects. It showed the lowest Qy transition energy, with its reddest absorption about 2 nm red-shifted, and the highest photostability under strong illuminations. Among the three isoforms, Lhcb 2 showed lowest thermal stability regarding energy transfer from Chl b to Chl a in the complexes, which implies that the main function of Lhcb 2 under high temperature stress is not the energy transfer. |
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Keywords: | Car carotenoid CD circular dichroism Chl chlorophyll DM d-maltoside" target="_blank">n-dodecyl-β-d-maltoside LHCIIb the major light-harvesting Chl a/b complexes of photosystem II Lhcb1 Lhcb2 and Lhcb3 different subunits of LHCIIb Lhcb1 Lhcb2 and Lhcb3 genes of the respective subunits of LHCIIb HPLC high-performance liquid chromatography PS photosystem Lut lutein Neo neoxanthin Vio violaxanthin Tm apparent dissociation temperature Tmf apparent dissociation temperature regarding energy transfer from Chl b to Chl a |
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