Using P MAS NMR to monitor a gel phase thermal disorder transition in sphingomyelin/cholesterol bilayers |
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| Authors: | Alison L Costello |
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| Institution: | Department of Nanostructured and Electronic Materials, Sandia National Laboratories, Albuquerque, NM 87185-0886, USA |
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| Abstract: | The impact of low cholesterol concentrations on an egg sphingomyelin bilayer is investigated using 31P magic angle spinning (MAS) NMR spectroscopy. The magnitude of the isotropic 31P MAS NMR line width is used to monitor the main gel to liquid crystalline phase transition, along with a unique gel phase pretransition. In addition, the 31P chemical shift anisotropy (CSA) and spin-spin relaxation times (T2), along with the effects of spinning speed, proton decoupling and magnetic field strength, are reported. The variation of this unique gel phase thermal pretransition with the inclusion of 5 through 21 mol% cholesterol is presented and discussed. |
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| Keywords: | SM sphingomyelin PC phosphatidylcholine Chol cholesterol MAS NMR magic-angle spinning nuclear magnetic resonance DSC differential scanning calorimetry CSA chemical shift anisotropy FWHM full width at half maximum |
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