艾纳香野生种群克隆多样性及克隆结构研究

艾纳香是具有克隆生长习性的多年生宿根性草本植物,其广布于中国南部,为了更有效地保护和合理利用艾纳香资源,本文利用RAPD分子标记技术,对4个野生艾纳香种群进行了克隆结构和克隆多样性(单克隆种群或多克隆种群)进行了初步研究。结果表明:(1)10对10bp随机引物共检测到70条谱带,其中多态带为60条,占85.71%,检测到64个基因型,且全部为局限基因型;(2)与Ellstrand Roose(1987)总结的克隆多样性平均值(PD=0.17,D=0.62)相比艾纳香的种群克隆多样性水平稍高,Simpson指数平均为0.973,基因型比率PD平均为0.800;(3)遗传一致度和遗传距离分析表明,4个艾纳香野生种群被分成两组,一组是海南的所有种群,另外一组是云南类群。

Clonal diversity and structure in natrual populations of Blumea balsamifera

Ramet of Blumea balsamifera belongs to perennial herbaceous plant,which widely distributes in south of China.The objectives of the present study were to assess the clonal structure and diversity(monoclonal or multiclonal population)on wild populations of B.balsamifera for the purpose of efficient conservation and reasonable utilization of its resources.Eighty individuals of B.balsamifera were collected from four natural populations in different and typical habitats in its major distribution areas in China,and they were studied byRAPD analysis.Seventy loci were detected by using 10 random primers(10bp),and 60 of them were found polymorphic(85.71%).Sixty-four RAPD genotypes were differentiated among the 80 plants sampled.The mean Simpson’s index was 0.973,and mean PD was 0.800,slightly higher than the mean of Ellstrand & Roose(PD=0.17,D=0.62).With UPGMA cluster analysis,four natural B.balsamifera populations can be divided into two clusters,one is all the Hainan populations,and the other is the Yunnan populaitions.