Development of diagnostic PCR markers closely linked to the tomato powdery mildew resistance gene Ol-1 on chromosome 6 of tomato |
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| Authors: | C C Huang Y-Y Cui C R Weng P Zabel P Lindhout |
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| Institution: | (1) Laboratory of Plant Breeding, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands Fax: +31-317-483457, NL;(2) Laboratory of Molecular Biology, The Graduate School of Experimental Plant Sciences, Wageningen, Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands, and the Department of Plant Pathology, College of Agriculture, Food & Natural resources, 108 Walters Hall, Plant Science Unit, University of Missouri-Columbia, Columbia, MO 65211, USA,;(3) Laboratory of Molecular Biology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands, NL |
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| Abstract: | Lycopersicon hirsutum G1.1560 is a wild accession of tomato that shows resistance to Oidium lycopersicum, a frequently occurring tomato powdery mildew. This resistance is largely controlled by an incompletely dominant gene Ol-1 near the Aps-1 locus in the vicinity of the resistance genes Mi and Cf-2/Cf-5. Using a new F2 population (n=150) segregating for resistance, we mapped the Ol-1 gene more accurately to a location between the RFLP markers TG153 and TG164. Furthermore, in saturating the Ol-1 region with more molecular markers using bulked segregant analysis, we were able to identify five RAPDs associated with the
resistance. These RAPDs were then sequenced and converted into SCAR markers: SCAB01 and SCAF10 were L. hirsutum-specific; SCAE16, SCAG11 and SCAK16 were L. esculentum-specific. By linkage analysis a dense integrated map comprising RFLP and SCAR markers near Ol-1 was obtained. This will facilitate a map-based cloning approach for Ol-1 and marker-assisted selection for powdery mildew resistance in tomato breeding.
Received: 21 June 1999 / Accepted: 1 December 1999 |
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| Keywords: | Resistance Tomato powdery mildew Tomato Mapping Oidium lycopersicum RFLP Sequence characterised amplified region (SCAR) |
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