GLYCOL SULPHATE ESTER FORMATION FROM [14C]NORADRENALINE IN BRAIN AND THE INFLUENCE OF A COMT INHIBITOR |
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| Authors: | R. F. Sugden D. Eccleston |
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| Affiliation: | M. R. C. Brain Metabolism Unit, Department of Pharmacology, University of Edinburgh, 1 George Square, Edinburgh, EH8 9JZ. |
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| Abstract: | A method is described allowing the identification and separation of the sulphate esters of the glycol metabolites of [14C]noradrenaline injected into the lateral ventricle of the rat. The esters of both the 3,4-dihydroxy derivative and the 3-methoxy-4-hydroxy derivative are formed in substantial amounts. It is suggested that the quantity of the former may have been underestimated in the past. Contemporaneous administration of pyrogallol, a catechol-O-methyl transferase inhibitor, with [14C] noradrenaline leads to a considerable fall in 3-methoxy-4-hydroxyphenylglycol sulphate without a rise in 3-4-dihydroxyphenylglycol sulphate although free 3-4-dihydroxyphenylglycol rises significantly. It is proposed that the latter may be an index of intraneuronal metabolism of noradrenaline and 3-methoxy-4-hydroxyphenylglycol that of released amine. |
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