L-Glutamate oxidase from Streptomyces cremeus 510 MGU: growth media optimization for the enhancement of the producer fermentative activity] |
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Authors: | K A Vinogradova I I Vlasova T S Sharkova |
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Institution: | Biological Faculty, M.V. Lomonosov Moscow State University. |
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Abstract: | The investigation was devoted to culture conditions optimization aimed to maximum secretion of extracellular L-glutamate oxidase by Streptomyces cremeus 510 MGU. It was shown that Ca ions at the concentration 5-20 mM and 0.1% ammonium sulphate enhanced activity of extracellular enzyme to 4 folds. L-glutamate acid supplement had no effect on enzyme activity. Influence of some bivalent cations and aeration regimes on L-glutamate oxidase activity was investigated. Growth media optimization along with screening of active variants resulted with isolation of the strain with L-glutamate oxidase activity about 2 U/mL Rate of peroxide degradation in the presence of filtrated culture of S. cremeus was determined by chemiluminescence method. |
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