A Chrome-Alum Preparation for Delicate and Difficult Fixations |
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Authors: | Frank Ammerman |
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Affiliation: | a Zoology Department, University of Michigan, |
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Abstract: | A chrome-alum fixative is recommended as a reagent for general use. The basic formula is: C.P. chrome-alum, 3 g.; 40% formaldehyde, 30 ml.; glacial acetic acid, 2 ml.; distilled water, 238 ml. This fixative permits easy sectioning of yolk-rich amphibian embryos. It can be used to make permanent slides of Euglena showing the flagellum. It is a satisfactory fixative for insect larvae and fixes sharply the slime droplets of Planarians. Fixation should not exceed two hours or the material being fixed will swell. Rinses of 70% alcohol or water may follow the fluid. The fluid keeps well, does not harden tissues and gives good cytological detail. |
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