Multiple resistance to sulfonylureas and imidazolinones conferred by an acetohydroxyacid synthase gene with separate mutations for selective resistance |
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Authors: | Jiro Hattori Robert Rutledge Hélène Labbé Douglas Brown Glen Sunohara and Brian Miki |
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Institution: | (1) Plant Research Centre, Agriculture Canada, K1A 0C6 Ottawa, Canada;(2) Present address: Petawawa National Forestry Institute, Forestry Canada, K0J 1J0 Chalk River, Ontario, Canada;(3) Present address: Biology Department, Bishop's University, JIM 1Z7 Lennoxville, Quebec, Canada |
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Abstract: | Summary The acetohydroxyacid synthase (AHAS) gene from the Arabidopsis thaliana mutant line GH90 carrying the imidazolinone resistance allele imr1 was cloned. Expression of the AHAS gene under the control of the CaMV 35S promoter in transgenic tobacco resulted in selective imidazolinone resistance, confirming that the single base-pair change found near the 3 end of the coding region of this gene is responsible for imidazolinone resistance. A chimeric AHAS gene containing both the imr1 mutation and the csr1 mutation, responsible for selective resistance to sulfonylurea herbicides, was constructed. It conferred on transgenic tobacco plants resistance to both sulfonylurea and imidazolinone herbicides. The data illustrate that a multiple-resistance phenotype can be achieved in an AHAS gene through combinations of separate mutations, each of which individually confers resistance to only one class of herbicides. |
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Keywords: | Acetohydroxyacid synthase Arabidopsis Imidazolinone Sulfonylurea Transgenic tobacco |
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