Sister chromatid exchanges induced by light flashes to 5-bromodeoxyuridine- and 5-iododeoxyuridine substituted Chinese hamster chromosomes |
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Authors: | T Ikushima S Wolff |
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Institution: | 1. Laboratory of Radiobiology, University of California, San Francisco, Calif. 94143, USA;2. Department of Anatomy, University of California, San Francisco, Calif. 94143, USA |
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Abstract: | Incorporation of 5-bromodeoxyuridine (BUdR) or 5-iododeoxyuridine (IUdR) into the chromosomal DNA of Chinese hamster ovary (CHO) cells during two rounds of replication causes sister chromatids to be differentiated so that they can be discriminated from one another by staining and morphology. Chromatids that contain BUdR or IUdR in both DNA strands stain lighter and are less condensed than their sister chromatids with only unifilar substitution. The halogenated pyrimidine nucleosides also induce sister chromatid exchanges that can be detected without autoradiography. The frequency of these exchanges is markedly increased by exposing the cells to light flashes. |
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