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The role of DNA polymerase I-associated 5'-exonuclease in replication of coliphage M13 replicative-form DNA.
Authors:S Dasgupta  S Mitra
Abstract:The conversion of both parental- and progeny-nascent open circular M13 RF DNA into covalently closed RF I is drastically reduced in an E. coli mutant deficient in the 5′ → 3′ exonuclease associated with DNA polymerase I. The nascent progeny RF DNA also contains a significant proportion of fragments of smaller than unit length.
Keywords:ssDNA  single-stranded DNA  RF DNA  replicative form DNA  pRF  parental RF  RF I DNA  covalently closed RF DNA  RF II DNA  RF DNA with one or more discontinuities in either or both strands
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