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Yeast-based protein delivery to mammalian phagocytic cells is increased by coexpression of bacterial listeriolysin |
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| Authors: | Walch Barbara Breinig Tanja Geginat Gernot Schmitt Manfred J Breinig Frank |
| Affiliation: | aMolecular and Cell Biology, Building A1.5, Saarland University, 66123 Saarbrücken, Germany;bJunior research group for Virology/Immunology, Department of Virology, Saarland University Hospital, 66421 Homburg, Germany;cInstitute for Medical Microbiology and Hygiene, Faculty for Clinical Medicine Mannheim of the University Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany |
| Abstract: | Yeast-mediated protein delivery to mammalian antigen-presenting cells is a powerful approach for inducing cell-mediated immune responses. We show that coexpression of the pore-forming protein listeriolysin O from Listeria monocytogenes leads to improved translocation of a proteinaceous antigen and subsequent activation of specific T lymphocytes. As the resulting yeast carrier is self-attenuated and killed after antigen delivery without exhibiting any toxic effect on antigen-presenting cells, this novel carrier system suggests itself as promising approach for the development of yeast-based live vaccines. |
| Keywords: | Saccharomyces cerevisiae T cell Listeriolysin O |
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