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Immobilization of thylakoids in porous particles and stabilization of the photochemical processes by glutaraldehyde action at subzero temperature |
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| Authors: | M. F. Cocquempot D. Thomas M. L. Champigny A. Moyse |
| Affiliation: | (1) Laboratoire de Technologie Enzymatique, E.R.A. no 338 du C.N.R.S., B.P. 233 U.T.C., F-60206 Compiègne, France;(2) Laboratoire de Physiologie Cellulaire Végétale, L.A. no du C.N.R.S., Université Paris-Sud, F-91405 Orsay, France |
| Abstract: | Summary Lettuce thylakoids were immobilized by the action of glutaraldehyde at sub-zero temperature in presence of albumin. Particles with sponge structure and good mechanical properties were obtained. The activity yield after immobilization was found equal to 70% for the oxygen production. Photosystems I and II were both active after immobilization. The yield for the ATP production was 27%. An increase of stability of the immobilized thylakoids was observed when stored under illumination. |
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