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Binding of a Transition State Analog to Newly Synthesized Rubisco
Authors:Boovaraghan Balaji  Michele Gilson  Harry Roy
Institution:(1) Biology Department, Rensselaer Polytechnic Institute, Troy, New York 12180-3590, USA;(2) Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USA
Abstract:Radioactive amino acids, when added to isolated pea chloroplasts or chloroplast extracts engaged in protein synthesis, are incorporated into Rubisco large subunits that co-migrate with native Rubisco during nondenaturing electrophoresis. We have added the transition state analog 2′-carboxyarabinitol bisphosphate (CABP) to chloroplast extracts after in organello or in vitro incorporation of radioactive amino acids into Rubisco large subunits. Upon addition of CABP the radioactive bands co-migrating with native Rubisco undergo a readily detected shift in electrophoretic mobility just as the native enzyme, thus demonstrating the ability of the newly assembled molecules to interact with this transition state analog.
Keywords:Chaperonin  Rubisco  Protein synthesis  Assembly
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