Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations |
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| Authors: | A R Kornblihtt M M Flawiá D de Mendoza G Glikin R Farías H N Torres |
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| Institution: | (1) Instituto de Investigaciones Bioquímicas ‘Fundación Campomar’, Fac. de Ciencias Exactas y Naturales, Universidad Nacional de Tucumán, Obligado 2490, 1428 Buenos Aires, Argentina;(2) Instituto de Química Biológica, Universidad Nacional de Tucumán, Obligado 2490, 1428 Buenos Aires, Argentina |
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| Abstract: | Summary Cytosolic adenylate cyclase activity from rat seminiferous tubules was purified by chromatography in DEAE-cellulose, hydroxylapatite
and Bio-Gel A-0.5 m as well as by centrifugation in sucrose gradients. In all these purification steps, fractions with adenylate
cyclase activity also contained binding activity for L-T3. Binding studies indicate the existence of two L-T3 receptor components associated to adenylate cyclase activity. The component exhibiting the highest hormone affinity has the
lowest binding capacity. |
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