The efficiency of 1M NH4Cl and 2 M NaCl for the isolation of pathogenic Nocardia from soil |
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Authors: | Aida van Gelderen de Komaid Estela Liliana Durán |
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Institution: | (1) Cátedra de Micología, Instituto de Microbiología Dr. Luis C. Verna , Facultad de Bioquimica, Quimica y Farmacia, Universidad National de Tucumán, Ayacucho 491, (4.000) San Miguel de Tucumán, Tucumán, República Argentina |
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Abstract: | The present paper reports an improvement to the classical method of the paraffin bait, by the usage of 1 M NH4Cl or 2 M NaCl to eliminate contaminant microflora of soil.The purpose is to introduce a change in the paraffin bait method in order to reduce time required to isolate pathogenic strains of Nocardia from their natural sources.For this study three main criteria were used: a) Determination of the inhibitory effect of different concentrations of salts on soil microflora; b) The tolerance of Nocardia brasiliensis, Nocardia asteroides and Nocardia caviae (Nocardia otitidis caviarum) strains to these chemical inhibitors; c) Determination of the efficiency of salts in the isolation of Nocardia from soil when strains are grown on paraffin baits. |
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