Alterations in tumour suppressor gene p53 in human gliomas from Indian patients |
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Authors: | Phatak Poornima Selvi S Kalai Divya T Hegde A S Hegde Sridevi Somasundaram Kumaravel |
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Institution: | (1) Department of Microbiology and Cell Biology, Indian Institute of Science, 560 012 Bangalore, India;(2) Manipal Institute for Neurological Disorders, Manipal Hospital, Airport Road, 560 017 Bangalore, India |
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Abstract: | Alterations in the tumour suppressorp53 gene are among the most common defects seen in a variety of human cancers. In order to study the significance of thep53 gene in the genesis and development of human glioma from Indian patients, we checked 44 untreated primary gliomas for mutations
in exons 5–9 of thep53 gene by PCR-SSCP and DNA sequencing. Sequencing analysis revealed six missense mutations. The incidence of p53 mutations
was 13⋅6% (6 of 44). All the six mutations were found to be located in the central core domain of p53, which carries the sequence-specific
DNA-binding domain. These results suggest a rather low incidence but a definite involvement of p53 mutations in the gliomas
of Indian patients. |
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Keywords: | Gliomas mutations p53 single strand conformation polymorphism |
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