Isolation,characterization, cloning and bioinformatics analysis of a novel receptor from black cut worm (Agrotis ipsilon) of Bacillus thuringiensis vip 3Aa toxins |
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| Authors: | Gamal H. Osman Raya Soltane Ibrahim Saleh Hussein H. Abulreesh Khaled S. Gazi Ibrahim A. Arif Ahmed M. Ramadan Hussien F. Alameldin Yehia A. Osman Mamdouh Idriss |
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| Affiliation: | 1. Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makka, Saudi Arabia;2. Microbial Genetics Department, Agricultural Genetic Engineering Research Institute (AGERI), Giza, Egypt;3. Faculty of Sciences of Tunis, Tunis El Manar University, Tunisia;4. Department of basic sciences, Adham University college, Umm Al-Qura University, Saudi Arabia;5. Prince Sultan Research Chair for Environment and Wildlife, Department of Botany & Microbiology, College of Sciences, King Saud University (KSU), Riyadh, Saudi Arabia;6. Department of Biology, Faculty of Arts and Sciences in Almandaq, Albaha University, Saudi Arabia;7. Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah 80203, Saudi Arabia;8. Plant Molecular Biology Department, Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt;9. Department of Energy - Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA;10. Department of Bioinformatics, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt;11. Microbiology Department, Faculty of Science, Mansoura University, Mansoura, Egypt;12. Department of Entomology, Faculty of Agriculture, Alexandria University, Egypt |
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| Abstract: | Black cutworm (BCW) is an economically important lepidopteran insect. The control of this insect by a Bt toxin and the understanding of the interaction between the Bt toxin and its receptor molecule were the objectives of this research work. A gene coding for a Vip3A receptor molecule was identified, characterized, and cloned, from the brush border membrane vesicles (BBMV) of the BCW. The nucleotide sequence analysis of the cloned putative Vip3A-receptor gene revealed that the gene was 1.3-kb long and exhibited no homology with any gene in the gene bank. We succeeded in identifying and characterizing most of the Vip3A-receptor gene sequence; and the nucleotide sequence analysis of the cloned putative Vip3A-receptor gene (accession no. KX858809) revealed about 92% of the expected sequence was recovered, which exhibited no homology with any gene in the GenBank. |
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| Keywords: | Corresponding authors at: Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah 21955, Saudi Arabia (G.H. Osman). Receptor Vip3A BBMV |
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