Physical and chemical mutagens improved Sporotrichum thermophile,strain ST20 for enhanced Phytase activity |
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| Authors: | Asim Mehmood Umal Baneen Ahmad Zaheer Muhammad Wasim Sajid Abrar Hussain Shahzad Saleem Qudsia Yousafi Hamid Rashid Hassan Riaz Awais Ihsan Farrukh Jamil Yasar Sajjad Nageena Zahid Muhammad Shahzad Anjam Muhammad Arshad Zeenat Mirza Sajjad Karim Mahmood Rasool |
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| Institution: | 1. Department of Biosciences, COMSATS University Islamabad, Sahiwal, Pakistan;2. Institute of Molecular Biology and Biotechnology, The University of Lahore, Defence Road, Lahore, Pakistan;3. Department of Biotechnology, COMSATS University Islamabad, Abbottabad Campus, Pakistan;4. Institute of Molecular Biology and Biotechnology (IMBB), Bahauddin Zakariya University, Multan, Pakistan;5. Department of Biotechnology, University of Okara, Pakistan;6. King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia;7. Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Saudi Arabia;8. Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia |
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| Abstract: | ObjectivePhosphorous is an essential micronutrient of plants and involved in critical biological functions. In nature, phosphorous is mostly present in immobilized inorganic mineral and in the fixed organic form including phytic acid and phosphoesteric compounds. However, the bioavailability of bound phosphorous could be enhanced by the use of phosphate solubilizing microorganisms such as bacteria and fungi. The phytases are widespread in an environment and have been isolated from different sources comprising bacteria and fungi.MethodologyIn current studies, we show the successful use of gamma rays and EMS (Ethyl Methane Sulphonate) mutagenesis for enhanced activity of phytases in a fungal strain Sporotrichum thermophile.ResultsWe report an improved strain ST2 that could produce a clear halo zone around the colony, up to 24 mm. The maximum enzymatic activity was found of 382 U/mL on pH 5.5. However, the phytase activity was improved to 387 U/ml at 45 °C. We also report that the mutants produced through EMS showed the greater potential for phytase production.ConclusionThe current study highlights the potential of EMS mutagenesis for strain improvement over physical mutagens. |
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| Keywords: | Corresponding author Gamma rays EMS mutagenesis Fungus Phosphate solubilizer |
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