Chemical fingerprint,acute oral toxicity and anti-inflammatory activity of the hydroalcoholic extract of leaves from Tocoyena formosa (Cham. & Schlecht.) K. Schum |
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Authors: | Francisco Rafael Alves Santana Cesário Thaís Rodrigues de Albuquerque Giovana Mendes de Lacerda Maria Rayane Correia de Oliveira Bruno Anderson Fernandes da Silva Lindaiane Bezerra Rodrigues Anita Oliveira Brito Pereira Bezerra Martins Jackson Roberto Guedes da Silva Almeida Mariana Lima Vale Henrique Douglas Melo Coutinho Irwin Rose Alencar de Menezes |
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Institution: | 1. Department of Chemical Biology, Molecular Bioprospecting Postgraduate Program, Regional University of Cariri, 63105-000 Crato, CE, Brazil;2. Natural Resources of the Semi-Arid Program, Federal University of the Vale São Francisco, Av. José de Sá Maniçoba, s/n, Campus Universitário, Petrolina, PE, Brazil;3. Department of Pharmacology, Federal University of Ceara, Avenue Av. da Universidade, 2853 - Benfica, Fortaleza, CE 60020-180, Brazil |
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Abstract: | Inflammation is a protective response of the organism against damaging agents, this process is considered beneficial, however in some situations, this response can be damage when exacerbated effect are present. This claim objective to evaluate the qualitative and quantitative chemical profile, acute toxic and anti-inflammatory effects of the hydroalcoholic extract of leaves from Tocoyena formosa (Cham. & Schlecht.) K. Schum. (HELTF). Quantitative and qualitative phytochemical analysis was performed by HPLC-DAD and colorimetric assay. The topical anti-inflammatory activity was determined in Croton oil-induced ear edema assay and systemic activity was performed in vascular permeability, paw edema induced by carrageenan and dextran. Phytochemical analysis of leaves from HELTF showed presence of tannin, flavonoid, saponins an other that confirmed by HPLC analysis. The extract did not cause significant with LD50 greater than 5000 mg/kg and did not promote significate reduction in topical inflammatory process. However, HELTF demonstrate significant reduction of paw edema induced by carrageenan and dextran. The HELTF (200 mg/kg) reduced the protein/cell migration in the intradermal carrageenan-induced inflammation. Our results demonstrated that the first time the chemical profile and describe the effective action in systemic anti-inflammatory, antiedematogenic activity and low acute toxicity. This activity presents, supporting its traditional use. However, new studies are necessary for the detection and clarification of the possible mechanism of action. |
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Keywords: | Corresponding author at: Laboratory of Molecular Chemistry and Pharmacology Department of Biological Chemistry Regional University of Cariri – URCA Crato-CE Rua Cel Antonio Luis 1161 Pimenta 63105-000 Brazil Acute toxicity Anti-inflammatory activity Chemical profile Tocoyena formosa |
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