Molecular detection of Histoplasma capsulatum indoors: A public health approach |
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| Authors: | Juliana Amorim-Conselheiro Lilian Dias Orico Maria Adelaide Galvão-Dias Iraci Martins Grigorio Hildebrando Montenegro Neto Adriana Ruckert Da Rosa Débora Cardoso Oliveira Carlos Pelleschi Taborda Adriana Araujo Reis-Menezes |
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| Institution: | 1. Laboratory of Zoonoses and Vector-borne Diseases, Zoonoses Surveillance Division – Health Surveillance Coordination, São Paulo, SP, Brazil;2. Chiroptera Section, Zoonoses Surveillance Division – Health Surveillance Coordination, São Paulo, SP, Brazil;3. Laboratory of Pathogenic Dimorphic Fungi, University of São Paulo – Institute of Biomedical Sciences, Department of Microbiology, São Paulo, SP, Brazil |
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| Abstract: | BackgroundHistoplasmosis, caused by the dimorphic fungus Histoplasma capsulatum, represents an important public health problem, especially in urban environments where bats and humans cohabit indoors.AimsTo detect the presence of H. capsulatum indoors, using samples of bat droppings collected in roost sites inside houses.MethodsA Real-Time TaqMan PCR assay targeting the ITS1 region of the ribosomal DNA of H. capsulatum was carried out.ResultsFifty-nine sampling points in the municipality of São Paulo were inspected, all of them located at inhabited places. H. capsulatum was isolated from nine samples.ConclusionsThe rapid identification and monitoring of sites where the fungus is present may contribute to make a more reliable database of H. capsulatum distribution. |
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| Keywords: | Corresponding author Histoplasmosis Bats Indoors Surveillance qPCR Histoplasmosis Murciélagos Interiores Vigilancia qPCR |
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