Isolation,characterization, and effect of phosphate-zinc-solubilizing bacterial strains on chickpea (Cicer arietinum L.) growth |
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Authors: | Ahmad Zaheer Arif Malik Ahmad Sher Muther Mansoor Qaisrani Asim Mehmood Sami Ullah Khan Muhammad Ashraf Zeenat Mirza Sajjad Karim Mahmood Rasool |
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Institution: | 1. Institute of Molecular Biology and Biotechnology, The University of Lahore, Defence road, Lahore, Pakistan;2. College of Agriculture, Bahauddin Zakariya University, Bahadur sub campus, Layyah, Pakistan;3. Department of Bioinformatics, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, Pakistan;4. Department of Biosciences, COMSATS University Islamabad, Sahiwal campus, Sahiwal 57000, Pakistan;5. Department of Botany, Women University of Azad Jammu & Kashmir Bagh, Pakistan;6. King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia;7. Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Saudi Arabia;8. Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia |
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Abstract: | ObjectivePhosphate (P) and zinc (Zn) are essential plant nutrients required for nodulation, nitrogen-fixation, plant growth and yield. Mostly applied P and Zn nutrients in the soil are converted into unavailable form. A small number of soil microbes have the ability to transform unsolvable forms of P and Zn to an available form. P-Zn-solubilizing rhizobacteria are potential alternates for P and Zn supplement. In the present study, the effect of two P-Zn-solubilizing bacterial strains (Bacillus sp. strain AZ17 and Pseudomonas sp. strain AZ5) was evaluated on the growth of chickpea plant.MethodologyBoth strains were purified from the rhizospheric soil of chickpea plant grown-up in sandy soil and rain-fed area (Thal desert). In vitro, both strains solubilize P and Zn as well both strain produce IAA and organic acids. In the field experiments, conducted in the rain-fed area, the positive influence of inoculation with both bacterial isolates AZ5 and AZ17 on chickpea growth was observed.ResultsThe application of inoculum (strains AZ5 and AZ17) resulted in up to 17.47% and 17.34% increase in grain yield of both types of chickpea grown in fertilized and non-fertilized soil, respectively over non-inoculated control. Strain AZ5 was the most effective inoculum, increasing up to 17.47%, 16.04%, 26.32%, 22.53%, 26.12% and 22.59% in grain yield, straw weight, nodules number, dry weight of nodules, Zn uptake and P uptake respectively, over control.ConclusionThese results indicated that Pseudomonas sp. strain AZ5 and Bacillus sp. strain AZ17 can serve as effective microbial inocula for chickpea, particularly in the rain-fed area. |
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Keywords: | Corresponding author Organic acids IAA Rain-fed |
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