Assessment of genetic relatedness among commercial and wild strains of Agaricus bisporus using repetitive extragenic palindromic sequences and polymerase chain reaction |
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Authors: | Nader Rokni Seyed Ali Akbar Sadati Naser Safaie Mohammad Ali Ebrahimi Mohammad Samimifar |
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Institution: | 1. Faculty of Science, Jundi-Shapur University of Technology, Dezful, P.O. Box 64615-334, Iran;2. Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, P.O. Box, 149771-3111, Iran;3. Department of Biotechnology, Payame Noor University, Tehran, P.O. Box 4697-19395, Iran |
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Abstract: | The genetic relatedness among 18 strains of Agaricus bisporus was assessed based on the fragment pattern analysis obtained by the amplification of genomic DNA by BOX, ERIC (ERICIR-I/ERIC2) and REP (REP1RI/REP2I) gene sequences. Based on the banding patterns of PCR-amplified products, eight putative groups among the 18 commercial and wild strains were recognized. REP-PCR generated multiple distinct products showing considerable variability among the strains with ERIC and REP elements successfully enabled detection of wild and commercial A. bisporus. Strains originating from the same geographical location were not always genetically related. To our knowledge, this was the first relevance study of biodiversity in commercial and native populations of A. bisporus by using the REP-PCR technique. The results confirmed the usefulness REP-PCR typing in intraspecific genetic variation assessments of the button mushroom. High level of Iranian wild strains distance with the commercial cultivars approves their importance as a promising new source of diversity in A. bisporus breeding program. |
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Keywords: | Corresponding author Button mushroom Genetic diversity Polymorphism REP-PCR typing |
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