基于SSLP分子标记验证遗传学三大定律的教学实践探索与体会

文章对“水稻SSLP分子标记的遗传分析”作为遗传学实验教学案例的实施过程及其效果进行了探讨。利用位于水稻两条染色体上的3个SSLP标记,对两亲本及其杂交构建的F₂代群体进行单株SSLP标记基因型检测,利用检测所得到的基因型结果验证分离定律、独立分配定律及连锁交换定律等遗传学三大定律。实践证明这不仅有利于加深学生对遗传学三大定律的认识,而且在提高学生实验操作技能和综合分析能力的基础上,还有助于培养学生的科研兴趣和创新意识。同时,对该实验的适用范围以及尚需完善之处做了讨论。此综合性实验也是科研成果转化为本科实验教学的一个有益探索。

Teaching practice and experiences of verifying the three laws of genetics based on the SSLP marker analysis

We explored the practical effect of the genetic analysis of simple sequence length polymorphism (SSLP) molecular markers in rice in the genetics lab course. Two parents and their F₂ population were analyzed and detected with three SSLP molecular markers that located on two chromosomes of the rice genome. The markers’ genotype data were used to verify the three laws of genetics, including segregation, independent assortment and linkage and crossing-over. Our practice has proved not only beneficial to deepen students’ understandings about the three laws of genetics, but also conducive to cultivate students’ interests in research and innovation and improve their skills and comprehensive analysis abilities. At the same time, the application scope of the experiment was discussed. This comprehensive experiment is also useful for the transformation of scientific research achievements into undergraduate experimental teaching.