| 非水介质中辣根过氧化物酶（HRP）催化酚类底物聚合反应速度的影响因素 |
| |
| 引用本文： | 马林,孙旭东,董爱武,黄仲立,由得林,程玉华.非水介质中辣根过氧化物酶（HRP）催化酚类底物聚合反应速度的影响因素[J].中国生物化学与分子生物学报,1995,11(1):95-99. |
| |
| 作者姓名： | 马林 孙旭东 董爱武 黄仲立 由得林 程玉华 |
| |
| 作者单位： | 吉林大学酶工程实验室 |
| |
| 摘 要： | 利用荧光测活法研究了非水介质中不同的对位取代基团对酚类底物ＨＲＰ催化聚合的反应速度的影响，发现反应速度既同对位取代基团的空间位阻效应有关，又同反应中间态的自由基活性有关，两者共同影响反应速度。同时还考察了有机溶剂对反应速度的影响。
|
| 关 键 词： | 辣根过氧化物酶 非水相 对位取代酚 反应速度 |
| 收稿时间： | 1995-02-20 |
Factors Influencing the Rate of Coupling Phenols by HRP in Nonaqueous Media |
| |
| Ma,Lin,Sun,Xu-Dong,Dong,Ai-Wu Huang,Zhong-Li You,De-Lin,Cheng,Yu-Hua.Factors Influencing the Rate of Coupling Phenols by HRP in Nonaqueous Media[J].Chinese Journal of Biochemistry and Molecular Biology,1995,11(1):95-99. |
| |
| Authors: | Ma Lin Sun Xu-Dong Dong Ai-Wu Huang Zhong-Li You De-Lin Cheng Yu-Hua |
| |
| Institution: | (The Lab of Enzyme Engineering, Jilin University, Changchun 130023 |
| |
| Abstract: | The effect of p-Substituted group of phenols on their coupling ratc was investigated in the present paper by fluorescence assay.The results indicated that the reaction rate for different phenols was affected by both activity and conformation of the p-substituted group. The effect of various organic solvents on the reaction rate was investigated also in this papaer. |
| |
| Keywords: | HRP Nonaqueous p-Substituted residue Reaction rate |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
| 点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文 |
