| Abstract: | The compound alpha-D-mannopyranosylmethyl-p-nitrophenyltriazene (alpha-ManMNT) has been tested for its effect on four alpha-D-mannosidase activities present in rat liver. When p-nitrophenyl alpha-D-mannopyranoside was used as a substrate, preincubation of enzyme with 1.0 mM alpha-ManMNT inhibited soluble alpha-D-mannosidase by 90%, lysosomal alpha-D-mannosidase by approx. 60%, and had virtually no effect on Golgi mannosidase II. Golgi mannosidase I removal of the four alpha-1,2-linked D-mannoses from the common Man9GlcNAc2 oligosaccharide structure formed during N-linked glycoprotein biosynthesis was also blocked by treatment of the Golgi fraction with this compound. Mannosyltriazene inhibition of the three susceptible hepatic alpha-D-mannosidases was largely irreversible. alpha-ManMNT should therefore be useful for studying oligosaccharide processing and possibly for determining the turnover time of the inhibited alpha-D-mannosidases. |
