| Abstract: | Murine lymphocytes, neutrophils, macrophages and eosinophils were assayed for lysophospholipase in order to determine the cellular source of the enzyme. The eosinophil was the only cell that demonstrated a positive reaction for the enzyme. The role of other cells and/or antigen in production of the enzyme by the eosinophil was also investigated. Results demonstrated that eosinophils cultured with both Trichinella spiralis antigen and other leukocytes (lymphocytes and/or macrophages) yielded enzyme activities significantly greater than did eosinophils cultured alone or with only antigen. More specific experiments showed that T-lymphocytes were the cells responsible for influencing the eosinophils' production of lysophospholipase in the presence of antigen, and that their influence was enhanced by the presence of macrophages. These results suggest that increased lysophospholipase activity present in parasitized tissue is not only due to an increased number of eosinophils infiltrating parasitized tissues, but is also due to each eosinophil synthesizing more of the enzyme for release. The necessity for antigen and other cells suggests a need for cell cooperation in the production of the enzyme, specifically T-lymphocytes and macrophage interaction with the eosinophil. |
