Molecular cloning and nucleotide sequence of human pancreatic secretory trypsin inhibitor (PSTI) cDNA |
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| Authors: | T Yamamoto Y Nakamura J Nishide M Emi M Ogawa T Mori K Matsubara |
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| Affiliation: | 1. Instituto de Biologia, Departamento de Biologia Vegetal, Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil;2. Sorbonne Universités, UPMC Univ Paris 06, Centre National de la Recherche Scientifique (CNRS), Laboratoire Jean Perrin (UMR 8237), 4 place Jussieu, 75252 Paris cedex 05, France;1. School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China;2. The Quartermaster Equipment Institute of General Logistcs Department of People’s Liberation Army, Beijing, China;3. Zhengzhou Mindtek Biotechnology Co. Ltd, Zhengzhou, Henan, China;1. Clinical Laboratory Department, Third Central Clinical College, Tianjin Medical University, 22, Qixiangtai Road, Heping District, Tianjin 300070, PR China;2. Clinical Laboratory Department, Tianjin Third Central Hospital, 83, Jintang Road, Hedong District, Tianjin 300170, PR China |
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| Abstract: | We have isolated and sequenced a cDNA clone coding for the human pancreatic secretory trypsin inhibitor (PSTI) from a human pancreatic cDNA library. The predicted product consists of 79 amino acids, and contains no apparent functional polypeptide other than PSTI. Southern blot analysis suggests that there is one copy of PSTI gene per haploid genome. This gene seems to be expressed not only in pancreas, but also in gastric mucosa, since a Northern blot analysis demonstrated the presence of a poly(A) RNA of the same size as in the pancreas. A comparison of sequences between human PSTI mRNA and mouse epidermal growth factor (EGF) mRNA revealed a high homology, suggesting that they share a common ancestral DNA sequence. |
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