Caged ATP - an internal calibration method for ATP bioluminescence assays |
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| Authors: | Calvert R M Hopkins H C Reilly M J Forsythe S J |
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| Institution: | Department of Life Sciences, The Nottingham Trent University, Nottingham and Biotrace Ltd, Bridgend, UK. |
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| Abstract: | ATP bioluminescence, based on the firefly luciferase system, is used for the rapid determination of hygienic practices in the food industry. This study has demonstrated the use of caged ATP as an internal ATP standard and quantified the effects of industrial cleansing solutions, alcoholic beverages and pH on firefly luciferase activity. The light signal was quenched 6-47% by five cleansing solutions at standard working concentrations. Ethanol at 1% (v/v) inhibited bioluminescence by 15% (w/v) whereas concentrations above 4% enhanced the light output. The light signal was quenched by 20-25% at pH values below pH 4 and above pH 10. |
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