HIF-1α/ERK通路活化调控微波长期辐射致大鼠海马神经元线粒体损伤

本文旨在探讨微波辐射致大鼠海马神经元线粒体损伤中HIF-1α和ERK通路分子表达的改变及意义,为深入研究微波辐射损伤机制和防治提供新靶标.2.5,5和10mW/cm2的微波辐射100只雄性Wistar大鼠,辐射时间为6min/次,5次/周,连续辐射1月,于辐射后6h,7d,14d,1周和2月,采用Real-timePCR,Westernblot和免疫组织化学检测海马中hif-1αmRNA,HIF-1α,ERK1/2和p-ERK1/2表达.结果发现,大鼠海马hif-1αmRNA和HIF-1α蛋白分别在2.5和5mW/cm2组于辐射后14d和1月明显增加,10mW/cm2组辐射后14d～2月降低.但海马ERK1/2未见明显改变.假辐射组p-ERK1/2于海马神经元胞浆中呈弱阳性,2.5mW/cm2组p-ERK1/2表达无明显变化,5和10mW/cm2辐射后7d～1月,p-ERK1/2于海马神经元胞浆和胞核中呈阳性或强阳性.2.5,5和10mW/cm2微波长期辐射后大鼠海马HIF-1α和p-ERK1/2表达的改变,表明HIF-1α和ERK通路活化参与微波辐射致海马线粒体损伤的过程,并可能发挥修复线粒体损伤的作用.

Activation of HIF-1α/ERK Pathway Regulates the Injury of Neuron Mitochondria in Hippocampus Induced by Long-term Microwave Exposure

Change of HIF-1α/ERK signaling pathway and its significance in the damage of the hippocampus induced by microwave exposure provides new targets for in-depth study of microwave injury mechanisms and prevention and control measures and approaches. 100 male Wistar rats were exposed to microwave at 2.5, 5 and 10 mW/cm². The exposure time was 6 min/times, 5 times/week, and the exposure was continuous for one month. Rats were sacrificed at 6 h, 7 d, 14 d, one and two months after exposure. The expression of hif-1α mRNA in hippocampus was detected by real-time PCR. HIF-1α, ERK1/2, p-ERK1/2 expression were detected by Western blot, IHC and image analysis. The mRNA and protein levels of HIF-1α in hippocampus were increased significantly at 14 d and one month in 2.5 and 5 mW/cm² groups, while decreased from 14 d to two months in 10 mW/cm² group. There was no significant change of ERK1/2 within two months in exposure groups. In sham group, p-ERK1/2 was expressed weakly positive in neuron cytoplasm of hippocampus, while there was no changes in 2.5 mW/cm² group, and p-ERK1/2 was expressed positive or strong positive from 7 d to one month in 5 and 10 mW/cm² group. Therefore, HIF-1α and p-ERK1/2 expression in hippocampus were changed after 2.5, 5 and 10 mW/cm² microwave exposure, which indicated the activation of HIF-1α and ERK pathway participated in the processes of hippocampus mitochondria injury induced by microwave, and might repair the mitochondria injury.