Expression of the Catalytic Subunit of Ouabain-Sensitive H+,K+-ATPase in Rat Skin Epidermis |
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Authors: | Romanova L G Novoselov S V Egorov M V Kostina M B Shakhparonov M I |
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Institution: | (1) Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997, Russia;(2) Russian Academy of Sciences, Institute of Cell Biophysics, Pushchino, Moscow oblast, 142292, Russia |
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Abstract: | A comparative localization of Na+,K+-ATPase and ouabain-sensitive H+,K+-ATPase in rat skin was performed using in situ RNA hybridization and immunohistochemistry. Na+,K+-ATPase was predominantly detected in the basal layer of the epithelium, whereas the ouabain-sensitive H+,K+-ATPase, in the granular and prickle cell layers. The genes of these ATPases are thus expressed in epithelial cells at different stages of their development. The hypothesis was advanced that the ouabain-sensitive H+,K+-ATPase is involved in maintaining the skin pH value. The probes specific to the mRNAs of the full-size -subunit of the ouabain-sensitive H+,K+-ATPase and its truncated form were used to establish a similar distribution of both mRNA variants in skin. |
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Keywords: | immunohistochemistry in situ RNA hybridization ouabain-sensitive H
+ K
+
-ATPase Na
+ K
+
-ATPase pH skin epithelium |
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