Direct gas chromatographic determination of dechloroethylcyclophosphamide following microsomal incubation of cyclophosphamide |
| |
| Authors: | Frank Bohnenstengel Susanne Johnson Ute Hofmann Michel Eichelbaum Heyo K. Kroemer |
| |
| Affiliation: | 1. Earth System Research Laboratory, Chemical Sciences Division, National Oceanic and Atmospheric Administration, Boulder, Colorado, USA;2. Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, Colorado, USA;3. Laboratory of Photochemistry and Chemical Kinetics, Department of Chemistry, University of Crete, Vassilika Vouton, 71003, Heraklion, Crete, Greece |
| |
| Abstract: | A method for the sensitive determination of dechloroethylcyclophosphamide (3-DCl) in microsomal incubation mixtures was developed. 3-DCl, a side-chain oxidation product of cyclophosphamide (CP), was isolated by extraction with acetic acid ethyl ester following solid-phase extraction on C8 cartridges. Quantification of the metabolite was performed by direct capillary gas chromatography with a nitrogen-phosphorus detector without prior derivatization. The method showed good sensitivity and reproducibility with a detection limit of 1 ng/ml and a limit of quantification of 5 ng/ml. The suitability of the method is shown for the quantification of 3-DCl following incubation of CP with human liver microsomes. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
