生物体衰老与复制衰老--体内与体外研究

体外连续培养的细胞在有人数的细胞分裂后,更新换代合成ＤＮＡ及分裂的能力,最后导致增殖能力的丧失,但基本代谢过程仍能维持,这种现象称为复制衰老。本文讨论了复制衰老现象存在的普遍性,描述了衰老细胞伯特征,对复制衰老和生物体衰老之间的联系进行了重点分析。现有的研究虽然还不完全,但都提示复制衰老是生物体衰老在细胞水平上的反映,并充分肯定了复制衰老是一个较好的研究机体衰老的模型。

Systemic aging and replicative senescence: in vivo and in vitro

Cultured cells lose the ability of DNA synthesis, mitosis, and finally the ability of cell proliferation after they have undergone a finite number of population doublings in vitro, though the cells still maintain the basic metabolic process. This is termed replicative senescence. We review the prevalence of replicative senescence, summarize the features of senescent cells, and then focus on the links between systemic aging and replicative senescence. The present knowledge, albeit still incomplete, proposes that replicative senescence is a reflection of systemic aging at cell level, and it fully confirms replicative senescence as a good model for the research of systemic aging.