|
|||||
|
|
Feasibility study for decomposition of gelatin layers on X-ray films by thermostable alkaline protease from alkaliphilic Bacillus sp. |
|
| Authors: | Akihiko Masui Nobuaki Fujiwara Masahiro Takagi Tadayuki Imanaka |
| Institution: | (1) Technology Research Institute of Osaka Prefecture, 2-7-1, Ayumino, Izumi, Osaka, 594-1157, Japan;(2) Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamadaoka, Suita, Osaka, 565-0871, Japan;(3) Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, 606-8501, Japan |
| Abstract: | Enzymatic decomposition of gelatin layers on X-ray films and repeated utilization of enzyme for potential industrialization were investigated using thermostable alkaline protease from the alkaliphilic Bacillus sp. B21-2. The decomposition of gelatin layers at 50 °C with the mutant enzyme (Ala187 was replaced by Pro) was higher than those of the wild-type and other mutant enzymes. In the repeated experiment for every 60 min (20 U ml–1, 50 °C), the mutant enzyme could be satisfactorily used five times while three times for the wild-type enzyme. |
| Keywords: | alkaline protease Bacillus sp gelatin protease protein engineering |
| 本文献已被 SpringerLink 等数据库收录！ | |