RNA结合蛋白QKI-5在前列腺癌中的表达分析

目的:检测前列腺癌及癌旁正常前列腺组织中RNA结合蛋白QKI-5的表达情况并分析其临床意义。方法:收集前列腺癌及与之匹配的癌旁组织124例,通过免疫组化染色、Western blot、实时PCR方法检测其QKI-5的表达水平,并分析QKI-5的表达与前列腺癌临床病理特征的关系。结果:前列腺癌组织中QKI-5蛋白及m RNA表达水平均明显低于癌旁正常前列腺组织,并且随着Gleason评分的增高而降低(P0.05)。前列腺癌组织中QKI-5的表达与其Gleason评分(r=-0.939,P0.05)、TNM分期(r=-0.913,P0.05)、血清PSA值(r=-0.743,P0.05)均密切相关。结论:QKI-5在前列腺癌的发生发展过程中可能起抑癌基因的作用,并可能作为前列腺癌的诊断、病情分析和预后评估的参考指标。

Analysis of the Expression of RNA-binding Protein QKI-5 in Prostate Cancer

Objective:To detect the expression of RNA-binding protein QKI-5 in prostate cancer and normal prostate tissue and analyze its clinical significance.Methods:The expression of RNA-binding protein QKI-5 in 124 cases of prostate cancer and matched adjacent tissues were detected by immunohistochemistry, western blot, real-time PCR, and the relationship between QKI-5 expression and clinicopathological features of prostate cancer patients was analyzed.Results:The protein and mRNA levels of QKI-5 in prostate cancer tissue were significantly lower than those in the adjacent normal prostate tissue, and with the increase in Gleason score, the expression of QKI-5 gradually was decreased (P<0.05). The expression of QKI-5 in prostate cancer was closely correlated with the Gleason score (r=-0.939, P <0.05), TNM staging (r=-0.913, P <0.05), serum PSA values ( r= -0.743, P <0.05).Conclusion:QKI-5 may act as a tumor suppressor gene in the development of prostate cancer, and could be used as a reference for the diagnosis, disease condition analysis and prognositic prediction of prostate cancer.