吴茱萸次碱软膏对小鼠尾部鳞片银屑病模型的治疗作用 |
| |
引用本文: | 石敏,石婷,向阳芳,陈菲,李岱.吴茱萸次碱软膏对小鼠尾部鳞片银屑病模型的治疗作用[J].现代生物医学进展,2015,15(28):5414-5417. |
| |
作者姓名: | 石敏 石婷 向阳芳 陈菲 李岱 |
| |
作者单位: | 中南大学湘雅医院药物临床试验机构;中南大学药学院;中南大学湘雅医学院医学检验系;珠海市人民医院 |
| |
基金项目: | 国家自然科学基金项目(81200047) |
| |
摘 要: | 目的:吴茱萸次碱(Rutaecarpa RUT)是芸香科植物吴茱萸的主要成分,它可激活辣椒素受体促进降钙素基因相关肽(CGRP)
等神经递质的释放来发挥药理作用。本实验的目的为观察吴茱萸次碱软膏对小鼠银屑病模型治疗作用。方法:通过药剂学方法制
成了不同浓度(2%, 5%, 10%)RUT 软膏,在小鼠尾部与背部银屑病模型给药14 天,观察小鼠尾部颗粒层形成的变化,给药结束后
取小鼠背部皮肤组织匀浆后采用放射免疫方法检测CGRP水平变化,并且取小鼠血浆检测CGRP水平变化。结果:不同浓度的吴
茱萸次碱软膏均能促进小鼠尾部颗粒层形成(P<0.05),并且浓度为5%与10%的RUT 软膏能显著降低小鼠背部CGRP 含量(P<0.
05),但是其对小鼠血浆的CGRP 没有影响。结论:吴茱萸次碱外用对小鼠银屑病模型有一定的治疗作用,这种作用与其促进小鼠
皮肤CGRP 释放而导致其含量降低有关。
|
关 键 词: | 银屑病 降钙素基因相关肽(CGRP) 吴茱萸次碱 治疗 |
Rutaecarpine Ointment in the Treatment of Psoriasis Model in Rats |
| |
Abstract: | Objective:Rutaecarpa (RUT) is the main component Rutaceae Evodia, which can activate the capsaicin receptor to
promote the release of calcitonin gene-related peptide (CGRP) and/or other neurotransmitters, in turn exert pharmacological effects. The
purpose of this experiment is to observe rutaecarpine ointment treatment effect on psoriasis mouse model and approach its mechanism.Methods:Different concentrations (2%, 5%, 10%) RUT ointments were prepared by pharmaceutical method. Mice tail particle layer
forming were chosen as psoriasis model, and mice skin of tail and back were administered by RUT ointments for 14 days to observe the
changes of mouse tail granular layer forming or skin CGRP levels. At the end of the administration mice back skin tissue homogenates
were determined CGRP levels using radioimmunoassay, and plasma of mice were taken to detect in CGRP levels.Results:Different
concentrations of rutaecarpine ointment promote mouse tail granular layer (P <0.05), and a concentration of 5% and 10% of the RUT
ointment can significantly reduce the back skin of mice CGRP content (P <0.05).Conclusion:Rutaecarpine exert therapeutic effect on
mouse psoriasis model, which is related to exhausting mouse skin CGRP. |
| |
Keywords: | Psoriasis Calcitonin gene-related peptide Rutacarpine Therapy |
本文献已被 万方数据 等数据库收录! |
| 点击此处可从《现代生物医学进展》浏览原始摘要信息 |
|
点击此处可从《现代生物医学进展》下载全文 |
|