The gut supports neurogenic differentiation of periocular mesenchyme, a chondrogenic neural crest-derived cell population |
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| Authors: | L C Smith-Thomas J P Davis M L Epstein |
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| Affiliation: | 1. The Murdoch Children''s Research Institute, Royal Children''s Hospital, Parkville 3052, Victoria, Australia;2. School of Mathematics and Statistics, The University of Melbourne, Parkville 3010, Victoria, Australia;3. Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany;1. School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China;2. Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China;3. Department of Orthopedic Surgery, Guangdong Provincial Hospital of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China;4. Department of Anatomy, Histology & Developmental Biology, School of Basic Medical Sciences, Shenzhen University Health Science Center, Shenzhen, China;5. School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China;6. College of Oceanology and Food Sciences, Quanzhou Normal University, Quanzhou, China;7. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China;8. Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China;9. Dr. Li Dak Sum Research Centre, The University of Hong Kong, Hong Kong, China;10. Faculty of Medicine, Macau University of Science and Technology, Macau, China;11. School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong, Hong Kong, China;12. Stem Cells and Regenerative Medicine, UCL Great Ormond Street Institute of Child Health, London, United Kingdom;13. Gastrointestinal Drug Discovery Unit, Takeda Pharmaceuticals International, Cambridge, Massachusetts |
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| Abstract: | Periocular mesenchyme (PM) is a mesencephalic neural crest derived cell population which as a result of an interaction with the retinal pigment epithelium forms the scleral cartilage of the avian eye. Enteric neurons are derived from vagal crest cells which invade the gut. To study factors which regulate neuronal differentiation, we investigated whether the gut could direct neurogenesis in PM, a cell population that does not produce neurons in vivo. We report here that PM cultured in the presence of aneural chick hindgut on the chorioallantoic membrane (CAM), invaded the gut and formed large numbers of neurons. These were localized in enteric ganglia and contained neurofilament immunoreactivity, vasoactive intestinal peptide immunoreactivity, and somatostatin immunoreactivity. In the control PM cultured alone on the CAM, a small number of cells contained neurofilament immunoreactivity but lacked the appearance of mature neurons. |
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