Generating somatic mosaicism with a Cre recombinase-microsatellite sequence transgene |
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Authors: | Akyol Aytekin Hinoi Takao Feng Ying Bommer Guido T Glaser Thomas M Fearon Eric R |
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Affiliation: | Department of Internal Medicine, University of Michigan Medical School, 109 Zina Pitcher, Ann Arbor, Michigan 48109, USA. |
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Abstract: | Strategies for altering constitutional or somatic genotype in mice are well established, but approaches to generate mosaic genotypes in mouse tissues are limited. We showed that a functionally inactive Cre recombinase transgene with a long mononucleotide tract altering the reading frame was stochastically activated in the mouse intestinal tract. We demonstrated the utility of this approach by inducing colonic polyposis after Cre-mediated bi-allelic inactivation of the Apc gene. |
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