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Site of integration of IS1-element responsible for expression of the yeast ADE1 gene in Escherichia coli |
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| Authors: | G K Gedminene K V Sasnauskas A A Ianula?tis |
| Abstract: | Cloned yeast ADE1 gene is expressed in Escherichia coli, due to integration of the IS1 bacterial element into the non-coding 5' region. Primary structure analysis of the integration site of IS1 element demonstrated that this site is situated in a region flanked by two inverted repeats of yeast DNA which are homologous to the right end of the IS1 element. The region up-stream (5') to the left inverted repeat is AT-rich. |
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