Molecular identification of co-occurring Cortinarius and Dermocybe species from southeastern Australian sclerophyll forests |
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Authors: | S M Chambers N A Sawyer J W G Cairney |
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Institution: | (1) Department of Biological Sciences,\nUniversity of Western Sydney (Nepean), P.O. Box 10,\nKingswood, NSW 2747, Australia\ne-mail: j.cairney@nepean.uws.edu.au, AU |
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Abstract: | The ability of restriction fragment length polymorphism (RFLP) analysis of the rDNA internal transcribed spacer (ITS) region
to discriminate 10 co-occurring Cortinarius and Dermocybe species at a southeastern Australian sclerophyll forest site was assessed. Using the basidiomycete-specific primers ITS1F
and ITS4B, some taxa were separated on the basis of individual RFLP patterns derived using the restriction endonucleases Hae III or Hinf I. Combined data from both endonucleases were, however, required to separate all taxa Dermocybe austro-veneta Clel. (Moser & Horak), C. rotundisporus Clel. & Cheel, C. archeri Berk., C. sinapicolor Clel., C. violaceus (L.: Fr.) S.F.Gray, C. radicatus Clel. and four morphologically-distinct, but unidentified Cortinarius spp.]. ITS sequence comparisons confirmed that D. austro-veneta belongs in Dermocybe, that C. rotundisporus is correctly placed in subgenus Phlegmacium, and suggest that Australian C. violaceus collections are not conspecific with northern hemisphere C. violaceus.
Accepted: 4 March 1999 |
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Keywords: | PCR RFLP ITS sequence analysis Ectomycorrhizal fungi Cortinarius taxonomy |
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