Walking, cloning, and mapping with yeast artificial chromosomes: a contig encompassing D21S13 and D21S16. |
| |
| Authors: | R Butler D J Ogilvie P Elvin J H Riley R S Finniear G Slynn J E Morten A F Markham R Anand |
| |
| Institution: | Biotechnology Department, ICI Pharmaceuticals, Macclesfield, Cheshire, United Kingdom. |
| |
| Abstract: | Chromosome 21 has often been used as a model system for the development of genome mapping and cloning strategies in humans. In this report methods for systematic chromosome walking, cloning, and mapping are exemplified in the construction of a 1.5-Mb yeast artificial chromosome (YAC) contig encompassing and extending 400 kb beyond each of the genetic loci D21S13 and D21S16. Isolation of insert-terminal sequences from YACs in this contig provides a set of closely spaced physical markers. These have been used to generate a long-range genomic restriction map. |
| |
| Keywords: | |
|
|
