| 水稻矮缩病毒外壳蛋白基因S8在昆虫细胞中的表达 |
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| 引用本文： | 李胜,刘慧君,陈章良,李毅.水稻矮缩病毒外壳蛋白基因S8在昆虫细胞中的表达[J].微生物学报,2001,41(2):162-166. |
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| 作者姓名： | 李胜 刘慧君 陈章良 李毅 |
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| 作者单位： | 北京大学生命科学学院,北京,100871 |
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| 基金项目： | 本研究由国家"863"项目资助(863-103-02-05及国家自然科学基金资助(39870027) |
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| 摘 要： | 将水稻矮缩病毒 (RiceDwarfVirus,RDV)外壳蛋白基因S8克隆到杆状菌毒转移载体 pVL1 393中 ,重组转移载体 pVL1 393 S8与线性杆状病毒RP2 3.LacZ共转染草地夜蛾细胞sf9,经过细胞体内同源重组、PCR筛选得到重组病毒RP2 3 S8。重组病毒RP2 3 S8感染sf9细胞后 ,收集细胞 ,并进行SDS PAGE及Western blot检测。结果表明 ,S8基因在昆虫细胞中成功表达 ,且在感染后 96h表达量最高
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| 关 键 词： | RDV 杆状病毒 昆虫细胞表达 Western-blot |
| 文章编号： | 0001-6209(2001)02-0162-05 |
| 修稿时间： | 2000年6月13日 |
EXPRESSION OF RICE DWARF VIRUS OUTER COAT PROTEIN GENE(S8) IN INSECT CELLS |
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| S Li,H Liu,Z Chen,Y Li.EXPRESSION OF RICE DWARF VIRUS OUTER COAT PROTEIN GENE(S8) IN INSECT CELLS[J].Acta Microbiologica Sinica,2001,41(2):162-166. |
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| Authors: | S Li H Liu Z Chen Y Li |
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| Institution: | Department of Biotechnology, College of Life Science, Beijing Univ., Beijing, 100871, China. |
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| Abstract: | Outer coat protein gene(S8) of RDV was cloned into the transfer vector pVL 1393 to construct a recombinant vector pVL1393-S8. The recombinant vector pVL1393-S8 and the linear baculovirus RP23. LacZ were cotransfected into sf9 cells to produce the recombinant virus RP23-S8. RP23-S8 infected sf9 cells were collected and analysed by SDS-PAGE and Western-blot. The results showed that the S8 gene of RDV was expressed in sf9 cells and the expression level of sf9 cells was higher between 72-96 h after infected. |
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| Keywords: | Rice Dwarf Virus Baculovirus Expression in insect cells Western blot |
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