The fermentation process for l-phenylalanine production using an auxotrophic regulatory mutant of Escherichia coli |
| |
Authors: | S. O. Hwang G. H. Gil Y. J. Cho K. R. Kang J. H. Lee J. C. Bae |
| |
Affiliation: | (1) R & D Center, Cheil Sugar & Co., Ltd., 522-1, Dokpyong-Ri, Majang-Myon, Ichon-Kun, Kyonggi-Do, Korea |
| |
Abstract: | Summary A process for l-phenylalanine production was studied using a tyrosine auxotrophic regulatory mutant of Escherichia coli, resistant to both -2-thienyl-dl-alanine and p-fluoro-dl-phenylalanine. Fermentations were carried out in a 30-1 fermentor with intermittent feeding of glucose plus phosphate. The mutant accumulated l-phenylalanine in the fermentation broth up to 15 g/l at pH 7.0 and 33°C. Column chromatography on a strong cation exchanger was employed as the most effective step in the purification of l-phenyl-alanine from the broth. This step brought about 4-fold concentration of the product with 96% recovery. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|